Open AccessInvolvement of DNA Polymerases I and III in the Replication of Bacteriophage M‐13
Author(s) -
STAUDENBAUER Walter L.
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03829.x
Subject(s) - dna polymerase , dna polymerase ii , dna clamp , polymerase , dna replication , dna polymerase i , microbiology and biotechnology , biology , dna synthesis , dna , dna polymerase delta , genetics , reverse transcriptase , gene , polymerase chain reaction
M‐13 replication was investigated in Escherichia coli mutants deficient in DNA polymerase I ( polA ) and DNA polymerase III ( dnaE ts ). The following results were obtained:1 M‐13 infection is abortive in both polA − and dnaE ts mutants at 42°C. 2 The conversion of the parental single‐stranded DNA to double‐stranded replicative forms (RF) is only slightly affected by the dnaE mutation. However, no parental double strands are formed in the polA − dnaE ts double mutant at the restrictive temperature, indicating a possible involvement of DNA polymerase I in this process. 3 The replication of the replicative‐form molecules as well as the synthesis of progeny single strands are inhibited by thermal inactivation of the dnaE function implying a requirement of DNA polymerase III in both types of DNA synthesis. 4 The presence of the polA mutation leads to an accumulation of open‐circular replicative forms. DNA polymerase I might, therefore, also be involved in the filling of gaps in the newly synthesized DNA molecules.