Open AccessCharacterization of DNA from Differentiated Cells
Author(s) -
Jiménez Estela Sánchez,
González José Luis,
Domínguez José Luis,
Saloma Enrique Sánchez
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03525.x
Subject(s) - dna , repeated sequence , genome , dna sequencing , microbiology and biotechnology , biology , denaturation (fissile materials) , chemistry , genetics , gene , nuclear chemistry
DNAs from different chicken tissues were purified. After denaturation, the reassociation reaction of two different molecular weight DNAs was measured by the hydroxyapatite method. The results of these experiments indicated that, under the generally accepted standard conditions, the chicken genome is comprised of 20%, repetitive DNA. Furthermore, it was found that a significant amount of the repetitive DNA was interdispersed among the unique DNA. It can be concluded from the kinetic data that there was not a significant difference either in the amount or in the distribution of the unique and repetitive sequences of the DNA samples tested. Isolation and further characterization of the repetitive sequences were carried out.