Open AccessRepeated Nucleotide Sequences in the Loach Genome
Author(s) -
Kupeijanova Natalia S.,
Timofeeva Margarita Ja.
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03457.x
Subject(s) - repeated sequence , genome , dna , nucleotide , biology , dna sequencing , genetics , kinetics , gene , physics , quantum mechanics
Reassociation kinetics of loach DNA has been studied. It has been shown that 50% of the loach genome consists of repeated nucleotide sequences. Most of them belong to an “intermediate” repetitive fraction of DNA, repetition frequency being equal to 10 4 −10 2 . Close study of the intermediate repetitive fraction of DNA reassociation kinetics reveals the existence of discrete repetitive DNA groups in the loach genome. The DNA fractions corresponding to discrete repetitive groups were isolated and their reassociation kinetics, nucleotide composition and thermal stability were studied. The differences established allow us to postulate that DNA sequences of different repetitive groups perform distinct functions. Further investigations are to be undertaken to examine this assumption and clarify the role of each repetitive group in the genome.