Open AccessStructural Investigations on the Salmonella T2 Lipopolysaccharide
Author(s) -
Bruneteau Maud,
Volk Wesley A.,
Singh Prem P.,
Lüderitz Otto
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03437.x
Subject(s) - substituent , chemistry , amino sugar , periodate , residue (chemistry) , sugar , glucosamine , stereochemistry , acid hydrolysis , hydrolysis , salmonella , polysaccharide , chemical structure , biochemistry , bacteria , organic chemistry , biology , genetics
Comparative chemical analyses have been performed on the lipopolysaccharides of a Salmonella T2 form and of a T2 − mutant derived from it and found to belong to the chemotype Ra. Methylation analysis showed that the T2 structure contains an N ‐acylglucosamine residue linked to position 4 of the subterminal glucose II unit of the core polysaccharide. The N ‐acyl group and a substituent which renders this glucosamine periodate resistant have not been identified. The structure of the T2‐specific region was further studied by the analysis of two fragments. One was obtained after Smith degradation and had the structure glucosaminyl‐1,4‐glucosyl‐glyceraldehyde, the amino sugar carrying the N ‐acyl group and the unknown substituent. The other fragment obtained after hydrazinolysis and acid hydrolysis was the trisaccharide GlcN‐1,4‐(GlcN‐1,2)‐Glc. The results show that the T2‐specific structure is represented by a substituted N ‐acylglucosamine unit which is attached to the core at the same position as the T1‐ and the O‐specific structures in the corresponding lipopolysaccharides. These results are in agreement with findings obtained with a T2, SR(4,12) form and the corresponding T2 − mutant.