Open AccessStepwise Dissociation of Histones from Rat‐Liver Chromatin in Alkaline Solutions
Author(s) -
Russev Georgi,
Venkov Christo,
Tsanev Roumen
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03407.x
Subject(s) - chromatin , histone , chemistry , dna , dissociation (chemistry) , biochemistry , biophysics , microbiology and biotechnology , biology , organic chemistry
Rat liver chromatin was sedimented in a sucrose density gradient under alkaline conditions. It is shown that in the pH interval 12.2—12.8 four histone species are dissociated stepwise in the sequence: F2b, F1, F2al and F3. Histone F2a2 remains bound to DNA even at pH 13. About 70% of the non‐histonc proteins dissociate from the chromatin at pH values lower than 12.2 and 30% remain bound to DNA in the range of pH studied.