Open AccessDetermination of Binding Parameters from Scatchard Plots
Author(s) -
Weder Hans Georg,
Schildknecht Jürg,
Lutz Rudolf A.,
Kesselring Paul
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03361.x
Subject(s) - scatchard plot , binding site , saturation (graph theory) , curve fitting , mathematics , chemistry , biological system , statistics , biology , biochemistry , combinatorics
Procedures for graphic and numerical parameter fitting from Scatchard plots for the case of two different independent sets of equivalent binding sites are described. These methods are compared with other procedures and their advantages and disadvatages are discussed. Estimates of how much one parameter can be changed without changing the Scatchard curve significantly are made. This variability of the parameters is discussed. It is shown that low saturation points are of considerable importance in evaluating the binding parameters of the high affinity binding site. An example of the parameter fitting procedures is given and the utility and limitations of the procedures are discussed.