Open AccessEffect of Adenosine on Methionyl‐tRNA Synthetase
Author(s) -
Lawrence Françoise
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03218.x
Subject(s) - methionine , transfer rna , biochemistry , adenosine , enzyme , trypsin , chemistry , adenosine triphosphate , non competitive inhibition , aminoacyl trna synthetase , stereochemistry , biology , rna , amino acid , gene
The methionyl‐tRNA synthetase activities were shown to be inhibited by adenosine. In the ATP⇋PP i exchange, and in the hydroxamate reactions, the inhibition was competitive with respect to ATP and uncompetitive with respect to L‐methionine. In the methionine transfer reaction, inhibition patterns are more complicated, however the inhibition tends to be competitive with respect to ATP at high methionine concentration. The inhibition by adenosine of the ATP⇋PP i exchange reaction has been observed for several aminoacyl‐tRNA synthetases (seryl‐, cysteyl‐, isoleucyl‐, valyl‐, phenylalanyl‐, methionyl‐ and trypsin‐modified methionyl‐tRNA synthetases). However under standard conditions the degree of inhibition varies from one enzyme to the other.