Open AccessIsolation and Characterisation of Two Calf‐Thymus Chromatin Non‐Histone Proteins with High Contents of Acidic and Basic Amino Acids
Author(s) -
Goodwin Graham H.,
Johns Ernest W.
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03188.x
Subject(s) - amino acid , chromatin , electrophoresis , chromatography , histone , chemistry , cellulose , polyacrylamide gel electrophoresis , biochemistry , amino acid analysis , ion chromatography , polyacrylamide , ion exchange , dna , ion , enzyme , organic chemistry , polymer chemistry
The group of calf thymus chromatin non‐histone proteins which have a high electrophoretic mobility have been fractionated by CM‐cellulose ion‐exchange chromatography. The fractions thus obtained have been characterised by polyacrylamide gel electrophoresis, amino acid analysis, N‐terminal amino acid analysis, and by their molecular weights. Using the CM‐cellulose chromatographic method, two proteins which have a high content of basic and acid amino acids have been isolated in a pure form.