Open AccessTransglycosylic Reactions of Nucleotides of Deoxy‐Sugars
Author(s) -
Zemek Jiří,
Kučár Štefan,
Bauer Štefan
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03185.x
Subject(s) - hexose , chemistry , glycogen , glycogen branching enzyme , moiety , substrate (aquarium) , nucleotide , glycogen synthase , biochemistry , yeast , stereochemistry , carbohydrate , uridine diphosphate glucose , kinetics , hexokinase , enzyme , biosynthesis , transferase , catalysis , glycolysis , biology , quantum mechanics , ecology , physics , gene
Incorporation of 3‐deoxy‐, 4‐deoxy‐, and 6‐deoxy‐analogues of d ‐glucose into glycogen was investigated in a reaction catalyzed with yeast UDPglucogen glucosyl transferase. It was found that hydroxyl groups at positions C‐3, C‐4 and C‐6 in the glucosyl moiety of the UDPG molecule are not essential for preserving its substrate properties in this transglycosylic reaction. The kinetics of 3‐deoxy‐, 4‐deoxy‐ and 6‐deoxy‐glucose incorporation into glycogen was compared with that of d ‐glucose and 2‐deoxy‐glucose. It was found that the affinity of the enzyme and the rate of hexose incorporation into glycogen decreases in following sequence: d ‐glucose, 2‐deoxy‐, 6‐deoxy‐, 4‐deoxy‐ and 3‐deoxyglucose. The deoxyglucosyl analogues of UDPG act as competitive inhibitors on the reaction of glycogen biosynthesis.