Open AccessStudies of Sugar Transport in Acholeplasma laidlawii
Author(s) -
Tarshis Mark A.,
Migoushina Valentina L.,
Panchenko Leonid F.,
Fedotov Nikolay S.,
Bourd Heinrich I.
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03182.x
Subject(s) - maltose , fructose , chemistry , carbohydrate , sugar , substrate (aquarium) , biochemistry , incubation , enzyme , sucrose , non competitive inhibition , biology , ecology
Different mechanisms of carbohydrate transport into Acholeplasma laidlawii cells have been studied. The following properties have been found.1 The glucose, maltose and fructose uptake is dependent on the time of incubation, the substrate concentration in the external medium, as well as on the pH and temperature, thus pointing to the enzymatic nature of carriers. 2 The carbohydrate uptake is inhibited by a number of energy inhibitors and uncouplers, thus pointing to the active nature of this proces. 3 The carbohydrate uptake is highly sensitive to SH‐inhibitors, characterising carriers as proteins. 4 The noncompetitive inhibition suggests that glucose and maltose are transported by single carrier involving two active centres.