Open AccessEffects of Deoxyribonucleases I and II on Rat‐Liver‐Chromatin Transcription in vitro
Author(s) -
Pays Etienne,
Ronsse Annie
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03175.x
Subject(s) - chromatin , dna , microbiology and biotechnology , biology , euchromatin , histone , transcription (linguistics) , biochemistry , chemistry , heterochromatin , linguistics , philosophy
1 The hydrolysis by DNAase I or II of DNA in chromatin remains limited even after prolonged incubation. 2 This limitation might be due to a redistribution under the action of DNAase of f l histone 3 With large amounts of DNAase, all of the chromatin DNA may however be digested. 4 A comparison is made between the extent of DNA hydrolysis by DNAase and the resulting inhibition of chromatin template activity for endogenous, Escherichia coli or rat liver (form II) RNA polymerases. Our results are not in favour of a greater sensitivity to DNAase for the euchromatic sequences than for the repressed ones. prolonged incubation. on chromatin DNA.