Open AccessTransfer of Valine into Rabbit Haemoglobin from Various Isoaccepting Species of Valyl‐tRNA Differing in Codon Recognition
Author(s) -
Takemoto Toshiyuki,
Takeishi Keiichi,
Nishimura Susumu,
Ukita Tyunosin
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03084.x
Subject(s) - valine , transfer rna , reticulocyte , ribosome , globin , biology , biochemistry , translation (biology) , escherichia coli , amino acid , microbiology and biotechnology , messenger rna , gene , rna
The transfer of valine into haemoglobin from various isoaccepting species of Val‐tRNA obtained from Escherichia coli and yeast, which differ in the specificity for codon recognition has been investigated using a rabbit reticulocyte cell‐free system. It was demonstrated that the Val‐tRNA species which bind to ribosomes in response to the codon GUG preferentially transferred valine into both α and β‐globin chains and that the other species of Val‐tRNA which cannot repond to GUG were scarcely utilized in the haemoglobin synthesis. Several possibilities to explain this phenomenon were examined and consequently it has been strongly suggested that valine codons in mRNA for rabbit globin, at least for α‐globin chain, are very rich in GUG and possibly GUA.