Open AccessAction of Protease I of Escherichia coli on RNA Polymerase of Same Origin
Author(s) -
Hermier Brigitte,
Pacaud Michèle,
Dubert JeanMarie
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03063.x
Subject(s) - escherichia coli , proteolysis , rna polymerase , protease , polymerase , chemistry , rna , biology , microbiology and biotechnology , biochemistry , enzyme , gene
The recently isolated protease I of Escherichia coli leads to the partial degradation of RNA polymerase of E. coli and causes, in particular, the splitting of subunits ββ′ into fragments of approximately the same size as that of factor σ. This action is similar to that of papaïn or α‐chymotrypsin employed for short periods at much lower concentrations. The immunochemical study presented leads to the identification of the origin of the fragments obtained by limited proteolysis and shown the lack of cross reaction between subunits ββ′ and σ factor.