Open AccessBacteriophage T7‐DNA Replication in vitro: an Experimental System
Author(s) -
Ferdinand Franz Josef,
Krause Elke,
Knippers Rolf,
Strätling Wolf
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb03046.x
Subject(s) - biology , bacteriophage , dna replication , dna , genome , virology , rolling circle replication , genetics , microbiology and biotechnology , gene , escherichia coli
We describe an experimental system which consists of intact double‐stranded T7 DNA and an unfractionated protein extract from bacteriophage‐infected cells. Under proper conditions, DNA replication is initiate. Evidence will be presented that, at least the viral DNA polymerase and the function of the viral gene‐4‐protein are required for initiation. Circumstantial evidence suggests that the viral RNA polymerasee is also necessary for initiation. After successful initiation a part of the viral genome is semiconservatively replicated.