Open AccessCharacterization of Five Neurotoxins Isolated from the Venoms of Two Elapidae Snakes Naja haje and Naja nigricollis
Author(s) -
Kopeyan Charles,
Rietschoten Jurphaas,
Martinez Gérard,
Rochat Hervé,
Miranda François,
Lissitzky Serge
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02831.x
Subject(s) - naja , elapidae , venom , cobra , chemistry , biology , biochemistry , computer science , programming language
Two neurotoxins were purified from Naja nigricollis venom using equilibrium chromatography. This method was shown to produce the most resolution to purify proteins having very close physico‐chemical properties. The toxins obtained were characterized, as well as three neurotoxins previously isolated from Naja haje venom, by the determination of N‐terminal (35 to 39 residues) and C‐terminal sequences. The usefulness of N‐terminal automatic sequencing for rapid identification of proteins is discussed.