Open AccessThe Inhibitory Effect of AMP on the Activation Reactions of the Amino Acids Involved in Gramicidin S Biosynthesis
Author(s) -
Kristensen Tom,
GilhuusMoe Carl Christian,
Zimmer TrineLise,
Laland Søren G.
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02793.x
Subject(s) - phenylalanine , gramicidin , amino acid , pyrophosphate , chemistry , biosynthesis , enzyme , gramicidin s , biochemistry , stereochemistry , inhibitory postsynaptic potential , biology , membrane , neuroscience
Kinetic studies show that AMP inhibits the activation of all the amino acids participating in the formation of gramicidin S. The activation was measured by the amino‐acid‐dependent ATP‐[ 32 P] pyrophosphate exchange reaction. AMP acts as a competitive inhibitor to ATP and a non‐competitive inhibitor to the amino acids. The large difference in the effect of AMP on the activation of l ‐phenylalanine and d ‐phenylalanine makes it probable that the activation of the two isomers takes place on different sites on the enzyme.