Open AccessDiamine Oxidase in Rabbit Liver
Author(s) -
ArgentoCerù Maria Paola,
Sartori Claudia,
Autuori Francesco
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02769.x
Subject(s) - diamine oxidase , fraction (chemistry) , microsome , chromatography , vesicle , chemistry , centrifugation , density gradient , sucrose , membrane , biochemistry , enzyme , physics , quantum mechanics
Subfractionation of the microsomes according to Dallner [4] showed that diamine oxidase is concentrated in the smooth microsomes. Centrifugation of the post‐mitochondrial supernatant treated with CsCl on a discontinuous three‐step gradient consisting of 0.8 M sucrose (density 1.12 g/ml) layered on 1.3 M sucrose (density 1.20 g/ml) resulted in three fractions: fraction 1 at the interface 1.034/1.12 g/ml; fraction 2 at the interface 1.12/1.20 g/ml; fraction 3 a pellet at the bottom of the tube. No diamine oxidase activity is present in fraction 1, but it is significantly more concentrated in fraction 2 than in fraction 3. Biochemical and morphological analysis of the subfractions revealed that fraction 1 mainly consists of plasma membrane fragments, fraction 2 of smooth vesicles and fraction 3 of rough vesicles and free ribosomes.