Open AccessAffinity Elution as a Purification Method for Aminoacyl‐tRNA Synthetases
Author(s) -
Haar Friedrich
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02731.x
Subject(s) - aminoacyl trna synthetase , elution , transfer rna , affinity chromatography , amino acyl trna synthetases , enzyme , ligand (biochemistry) , biochemistry , homogeneous , yeast , biology , chemistry , chromatography , rna , receptor , gene , physics , thermodynamics
Aminoacyl‐tRNA synthetases adsorbed on phosphocellulose can be specifically eluted with purified tRNA. This method we call affinity elution in analogy to affinity chromatography. The advantage of the method is the use of enzyme‐ligand affinity without fixation of the ligand to a polymer support. As an example, purification of phenylalanyl‐tRNA synthetase, valyl‐tRNA synthetase, seryl‐tRNA synthetase and isoleucyl‐tRNA synthetase from baker's yeast is described. The first two aminoacyl‐tRNA synthetases were obtained as apparently homogeneous proteins with purification factors 537 and 246, respectively.