Open AccessPurification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzae
Author(s) -
Vogt Volker M.
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02669.x
Subject(s) - nuclease , aspergillus oryzae , nucleic acid , dna , rna , chemistry , enzyme , aspergillus niger , biochemistry , aspergillus , micrococcal nuclease , biology , microbiology and biotechnology , chromatin , gene , nucleosome
A simple scheme has been devised for the purification of Aspergillus nuclease S 1 from commercially available crude amylase powder. Five purification steps yield 90% pure nuclease with an overall yield of 27%. The enzyme is shown to be absolutely specific for single‐stranded nucleic acids. It degrades both DNA and RNA. The nuclease exhibits a sharp pH optimum at pH 4.2 and is active in low concentrations of dodecylsulfate. Aspergillus nuclease S 1 is a metalloprotein of molecular weight 32000. The utility of this nuclease for measuring annealing of labeled DNA is discussed.