Open AccessAnalysis of Bacteriophage‐M 13‐DNA Replication in an Escherichia coli Mutant Thermosensitive in DNA Polymerase III
Author(s) -
Staudenbauer Walter L.,
OLSEN William L.,
Hofschneider Peter Hans
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02604.x
Subject(s) - dna polymerase , dna polymerase ii , dna polymerase i , dna replication , dna clamp , microbiology and biotechnology , biology , polymerase , dna synthesis , dna , bacteriophage , dna polymerase delta , mutant , escherichia coli , genetics , polymerase chain reaction , gene , reverse transcriptase
The various stages of M 13 DNA replication were investigated in an Escherichia coli dnaE mutant thermosensitive in DNA polymerase III after a shift to the restrictive temperature (45°C). The following results were obtained. (1) DNA polymerase III is not needed for the conversion of the infecting single‐stranded phage DNA into the parental replicative form. (2) Semi‐conservative replicative form replication is completely blocked in the absence of a functional DNA polymerase III. (3) Synthesis of phage‐specific DNA continues after a temperature shift late in the infection. However, due to an inherent thermosensitivity of M 13 single‐stranded circular DNA production no free single strands are detected.