Open AccessBiological Activities of Lipid A Complexed with Bovine‐Serum Albumin
Author(s) -
Galanos Chris,
Rietschel Ernst T.,
Lüderitz Otto,
Westphal Otto,
Kim Yoon B.,
Watson Dennis W.
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb02524.x
Subject(s) - lipid a , bovine serum albumin , escherichia coli , mutant , chemistry , albumin , salmonella , serum albumin , biochemistry , lipopolysaccharide , biological activity , microbiology and biotechnology , bacteria , biology , in vitro , immunology , genetics , gene
Lipid A preparations from the lipopolysaccharides of four Salmonella minnesota R mutants and one Escherichia coli 0100 R mutant were assayed as soluble complexes with bovine serum albumin for lethality in mice, pyrogenicity in rabbits and complement inactivation. Although generally less active than endotoxic lipopolysaccharides, these lipid · albumin complexes nevertheless exhibited strong biological activity. These results indicate that lipid A contains the endotoxic principle of bacterial lipopolysaccharides.