Open AccessComplex Formation between 3‐Phospho‐ d ‐glyceric Acid and Divalent Metal Ions
Author(s) -
Larsonraźnikiewicz Märtha
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb02129.x
Subject(s) - deprotonation , chemistry , potentiometric titration , glyceric acid , divalent , inorganic chemistry , dissociation (chemistry) , dissociation constant , ligand (biochemistry) , stoichiometry , metal , metal ions in aqueous solution , medicinal chemistry , acid dissociation constant , titration , ion , stereochemistry , catalysis , organic chemistry , aqueous solution , biochemistry , receptor
Stoichiometric dissociation constants of complexes between the fully deprotonated 3‐phospho‐ d ‐glyceric acid and Mg 2+ , Mn 2+ , Ca 2+ , Co 2+ , Zn 2+ , and Ni 2+ , respectively, were determined in 0.25 M NaCl at 25°C. Potentiometric titrations of the ligand with these divalent metal ions were performed over a pH range close to the pK for the final deprotonation step of the ligand. The observed pH changes were utilized to estimate the dissociation constants; both algebraic and graphical techniques were employed in the estimations. Under the experimental conditions 1:1 complexes were the predominant products.