Open AccessInteraction of Concanavalin A with Rat Lymphocytes
Author(s) -
Betel Ido,
BERG Kor J.
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb02128.x
Subject(s) - concanavalin a , membrane , binding constant , covalent bond , dna , binding site , lymphocyte , stimulation , microbiology and biotechnology , chemistry , cell , dna synthesis , biophysics , biochemistry , biology , immunology , in vitro , endocrinology , organic chemistry
1 Concanavalin A covalently bound to acrylic polymer particles (enzacryl AA), which cannot enter the cells, stimulated rat lymphocytes into DNA synthesis. 2 Evidence was obtained that concanavalin A binds to the lymphocyte membrane specifically through its sugar‐binding sites. No nonspecific adsorption was found. 3 The equilibrium constant found for the binding of concanavalin A to the cell was 12 μ −1 at 37°C and 3° −l at 0°C. No heterogeneity in the affinity of the binding sites was detected. The average maximum number of concanavalin A molecules (Mol. wt 108000) that could be bound was about 5 ×10 6 per cell. 4 Optimum stimulation of DNA synthesis was obtained when about 5%, of the available sites on the cells were occupied by concanavalin A.