Open AccessStudies on a Bromouracil‐Labelled DNA Fraction Induced by Antigenic Stimulation
Author(s) -
Souleil Claude,
Panijel Jacques
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb02091.x
Subject(s) - pronase , dna , chemistry , microbiology and biotechnology , biochemistry , antigen , guinea pig , biology , enzyme , trypsin , immunology , genetics
When primed guinea‐pig lymph‐node cells are incubated in vitro with homologous antigen and 5‐bromo‐2‐deoxy‐[ 3 H]uridine, a peak of intermediate density appears between hybrid and normal DNA. This peak does not result from the attachment of a low density substance to DNA, since it is still present in denaturation conditions or after extensive digestion by pronase or phospholipase. By contrast, it disappears after DNA breakage by sonication, indicating that it results from end‐to‐end joining of labelled and unlabelled DNA segments.