Open AccessThermochemistry of the Avidin‐Biotin Reaction
Author(s) -
Suurkuusk Jaak,
WadsÖ Ingemar
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb01930.x
Subject(s) - biotin , avidin , chemistry , enthalpy , ethanol , ammonium acetate , chromatography , biochemistry , thermodynamics , high performance liquid chromatography , physics
The enthalpy for the binding of biotin to avidin has been determined at 25, 34 and 43°C (pH 9, ammonium acetate buffer): Δ H (25°C) =–22.5 ± 0.1 kcal/mol biotin; Δ C p =–237 ± 12 cal × mol biotin −1 × K −1 . Solubilities and enthalpies of solution of biotin in water and in ethanol were determined at 25°C. The transfer process for biotin between water and ethanol was considered as a simple model for the protein binding process.