Open AccessEffects of Aldosterone and Other Steroids in vivo on Rat‐Kidney Cortex
Author(s) -
Trachewsky Daniel,
Majumdar Adhip. P. Nandi,
Congote L. Fernando
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb01797.x
Subject(s) - ribosome , aldosterone , in vivo , kidney , renal cortex , protein biosynthesis , in vitro , chemistry , medicine , transfer rna , endocrinology , polysome , biochemistry , biology , rna , microbiology and biotechnology , gene
An investigation of the effects of aldosterone, deoxycorticosterone, 9α‐fluorocortisol, and 17β‐estradiol administration, respectively, to adrenalectomized male rats on the polypeptide synthesis by and poly(U)‐directed binding of phenylalanyl‐tRNA to isolated renal cortical ribosomes has revealed several interesting facts. As well as causing an increase in the ability of the ribosomes to synthesize polyphenylalanine in the presence of poly(U), the three mineralcorticoids also increased the poly(U)‐directed binding of phenylalanyl‐tRNA to the ribosomes. 17β‐Estradiol treatment, however, caused a decrease in the ability of the ribosomes to incorporate phenylalanine into polyphenylalanine when compared to the untreated controls. There were also no significant differences in the amount of poly(U)‐directed binding of phenylalanyl‐tRNA to the ribosomes of the estrogen‐treated rats when compared to the untreated animals. Consequently, it was concluded that all four steroids had induced some alterations in the properties of the renal cortical ribosomes. The effect of aldosterone and 17β‐estradiol on the thiol content of the nuclear proteins from rat kidney cortex showed an interesting correlation with the translational effects of both steroids. Thus aldosterone increased, and 17β‐estradiol slightly decreased, the thiol content of these proteins, measured as the incorporation of radioactive iodoacetate in whole nuclei in vitro. Under the experimental conditions used, the reaction was especially concentrated in discrete protein fractions of molecular weights of about 14000 and 42000. The effect of the hormones on the thiol content was not specifically concentrated in any of these protein fractions.