Open AccessOn the Kinetics of the Reaction of Hemoglobin with CO
Author(s) -
Schmelzer Uwe,
Steiner Rudolf,
Mayer Adalbert,
Nedetzka Teja,
Fasold Hugo
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb01720.x
Subject(s) - hemoglobin , flash photolysis , chemistry , molecule , kinetics , flash (photography) , activation energy , photochemistry , reaction rate constant , organic chemistry , art , visual arts , physics , quantum mechanics
Flash‐photolysis measurements were performed on normal and on chemically modified human adult hemoglobin solutions in the concentration range between 1 to 100 μM (for complete molecule) and in the pH range between pH 5 and pH 9. The energy of the irradiating flash was varied continuously from 20 to 400 Joules. It is shown that the fast reaction observed after complete or partial flash photolysis is pertinent both to dimeric hemoglobin molecules and to tetrameric hemoglobin molecules which are locked in the state of quaternary structure corresponding to completely liganded hemoglobin. The data suggest that an all‐or‐none transition of the quaternary structure of hemoglobin accompanies the third step of the ligation process.