Open AccessThe Pathway of Gluconeogenesis in the Cortex of Guinea‐Pig Kidney
Author(s) -
Longshaw Ian D.,
Bowen Neil L.,
Pogson Christopher I.
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb01705.x
Subject(s) - gluconeogenesis , kidney cortex , guinea pig , phosphoenolpyruvate carboxykinase , mitochondrion , kidney , biology , cortex (anatomy) , biochemistry , citric acid cycle , medicine , endocrinology , chemistry , metabolism , enzyme , neuroscience
1 Aminooxyacetate is a potent inhibitor of glutamate: oxaloacetate transaminases from both rat and guinea pig kidney cortex. 2 In guinea pig kidney cortex, phospho enol pyruvate carboxykinase activity was found to be 59% cytoplasmic, 41% mitochondrial. 3 Mitochondrial phospho enol pyruvate carboxykinase activity is expressed only after sonication indicating that the enzyme is located within the inner mitochondrial membrane. 4 Aminooxyacetate inhibits gluconeogenesis from lactate, but not from pyruvate, in both rat and guinea‐pig kidney cortex. 5 It is concluded that gluconeogenesis in guinea‐pig kidney cortex proceeds by a pathway involving cytoplasmic, rather than mitochondrial phospho enol pyruvate carboxykinase.