Open AccessIncorporation of 5‐Iodocytidine into Yeast tRNA Phe with tRNA Nucleotidyl Transferase in vitro
Author(s) -
Sprinzl Mathias,
Haar Friedrich,
Schlimme Eckhard,
Sternbach Hans,
Cramer Friedrich
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb01692.x
Subject(s) - transfer rna , transferase , yeast , chemistry , biochemistry , in vitro , enzyme , rna , gene
5‐Iodocytidine (io 5 C) is incorporated into tRNA Phe ‐A 73 as well as into tRNA Phe ‐A 73 ‐C 74 using tRNA nucleotidyl transferase. The tRNA Phe ‐A 73 ‐C 74 ‐io 5 C 75 ‐A 76 and tRNA Phe ‐A 73 ‐io 5 C 74 ‐io 5 C 75 ‐A 76 , respectively, thus obtained can be aminoacylated by the corresponding synthetase exhibiting the same K m but a slightly lowered V compared to tRNA Phe ‐A 73 ‐C 74 ‐C 75 ‐A 76 . An analytical method is presented which enables quantitative determination of the 3′ terminal nucleosides.