Open AccessSynthesis and Properties of Poly(s 2 C), a new Poly(C) Analog
Author(s) -
Scheit KarlHeinz,
Faerber Peter
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb19697.x
Subject(s) - circular dichroism , optical rotatory dispersion , polynucleotide phosphorylase , chemistry , phosphorolysis , polynucleotide , absorption (acoustics) , cotton effect , absorption spectroscopy , crystallography , ultraviolet , hydrolysis , enzyme , organic chemistry , materials science , purine nucleoside phosphorylase , biochemistry , physics , optoelectronics , quantum mechanics , purine , composite material
Polymerisation of 2‐thiocytidine‐5′‐diphosphate (s 2 CDP) by polynucleotide phosphorylase from Escherichia coli leads to the formation of poly(s 2 C) which has been characterized by ultracentrifugation, enzymatic hydrolysis and phosphorolysis. The ultraviolet absorption derivative, optical rotatory dispersion and circular dichroism spectra of 2‐thiocytidine and poly(s 2 C) have been measured. Evidence for n ‐π* transitions in the absorption spectra of these compounds is derived from derivatives of ultraviolet absorption and circular dichroism spectra. The absorption‐temperature profiles of poly(s 2 C) in the acid pH range prove the existence of double‐stranded structures which are much less stable than those of poly(C). From these observations it is concluded that hydrogen bonding predominantly controls the stability of the poly(C) helix under acidic conditions.