Open AccessHeterogeneity and Polymorphism of Human‐Liver Alcohol Dehydrogenase
Author(s) -
Schenker Toni M.,
Teeple Leslie J.,
Wartburg JeanPierre
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb19681.x
Subject(s) - isozyme , alcohol dehydrogenase , enzyme , biochemistry , microbiology and biotechnology , chemistry , biology , human liver , polymorphism (computer science) , allele , gene
1 Seven isoenzymes of human liver alcohol dehydrogenase have been ascertained by means of starch gel electrophoresis and chromatography on ion‐exchange celluloses. Six of them have been isolated and purified. 2 Catalytic properties of the isolated isoenzymes and the results of their reversible monomerization suggest that they are formed by the possible dimeric combinations of three subunits A, B and B′. 3 The same isoenzymes as observed in normal livers have been found in livers containing the atypical, variant enzyme described previously. The predominating isoenzyme isolated from atypical livers exhibits the catalytic features characteristic for the atypical enzyme in liver homogenates. One isoenzyme with normal kinetic properties, however, is also found in atypical livers.