Open AccessThe Identification of 3‐ O ‐Methyl‐D‐xylose and 3‐ O ‐Methyl‐L‐xylose as Constituents of the Lipopolysaccharides of Myxococcus fulvus and Rhodopseudomonas viridis , respectively
Author(s) -
Weckesser Jürgen,
Rosenfelder Günter,
Mayer Hubert,
Lüderitz Otto
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb19660.x
Subject(s) - xylose , rhodopseudomonas , chemistry , rhodopseudomonas palustris , bacteria , rhodospirillales , biochemistry , biology , fermentation , enzyme , photosynthesis , genetics
Lipophilic sugar constituents with identical chromatographic properties were identified in the lipopolysaccharides of the two gram‐negative bacteria, Myxococcus fulvus (Myxococcaceae) and Rhodopseudomonas viridis (Rhodospirillaceae). Both sugars were characterized as 3‐ O ‐methylpentoses by mass spectrometry, and xylose was obtained after demethylation. The optical rotation of the O ‐methyl sugars and the reactivity of the demethylated sugars with D‐glucose oxidase revealed that Myxococcus fulvus contains 3‐ O ‐methyl‐D‐xylose whereas Rhodopseudomonas viridis contains 3‐ O ‐methyl‐L‐xylose.