Open AccessA DNA Polymerase Induced by Bacteriophage T7
Author(s) -
Oey Jan L.,
StrÄTLING Wolf,
Knippers Rolf
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01646.x
Subject(s) - bacteriophage , dna polymerase , polymerase , primer (cosmetics) , enzyme , dna , dna polymerase i , chemistry , dna clamp , microbiology and biotechnology , polymerase chain reaction , biochemistry , biology , escherichia coli , reverse transcriptase , gene , organic chemistry
A bacteriophage‐induced DNA polymerase was purified from T7‐infected bacteria. This enzyme is composed of one single polypeptide chain with a molecular weight of about 1. The enzyme requires single‐stranded DNA with a 3′‐OH end as a primer. In addition to its polymerising activity the enzyme has a 3′‐exonucleolytic activity.