Open AccessDetermination of the Stability Constant of the Megnesium‐Ion Complex of Isocitrate
Author(s) -
Londesborough John C.,
Dalziel Keith
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01608.x
Subject(s) - chemistry , titration , ion , magnesium , stability constants of complexes , chloride , constant (computer programming) , inorganic chemistry , analytical chemistry (journal) , chromatography , organic chemistry , computer science , programming language
The stability constant of the magnesium‐ion complex of isocitrate has been measured by spectrophotometric and fluorimetric titrations of 8‐hydroxyquinoline with magnesium chloride in the presence and absence of isocitrate. Values of 195 ± 15M −1 at 19 °C, and 25°C, and 217 ± 10M −1 at 37°C were obtained at pH 7.0 and ionic strengh 0.15.