Open AccessA Novel Form of Initiation Factors from Escherichia coil which Binds Formylmethionyl tRNA and GTP: “F2 · F3 Complex”
Author(s) -
Groner Yoram,
Revel Michel
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01525.x
Subject(s) - gtp' , ternary complex , ribosome , transfer rna , escherichia coli , chemistry , biophysics , biology , biochemistry , crystallography , rna , enzyme , gene
A fraction containing a complex between initiation factors F2 and F3 was isolated from Escherichia coli ribosomes. In contrast to the free factors, this F2 · F3 complex makes a stable ternary association with formylmethionyl‐tRNA and GTP. F2 · F3 complex can be formed on the ribosomes during the process of initiation. It acts preferentially on the 30 S ribosomal subunit to which it confers a much larger affinity for fMet‐tRNA than observed with the free factors. A model for the function of F2 · F3 complex as an intermediate in the initiation cycle is proposed.