Open AccessRelease of Ethanolamine Pyrophosphate during Mild Acid Hydrolysis of the Lipopolysaccharide of Pseudomonas aeruginosa
Author(s) -
Drewry David T.,
Gray George W.,
Wilkinson Stephen G.
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01483.x
Subject(s) - ethanolamine , chemistry , pyrophosphate , hydrolysis , sephadex , chromatography , phosphate , hydrolysate , biochemistry , enzyme
Low molecular weight solutes released during mild acid hydrolysis of the lipopolysaccharide of Pseudomonas aeruginosa were isolated from the fraction containing the partially degraded polysaccharide, by successive chromatography on columns of Sephadex G‐75 and G‐10, followed (as necessary) by preparative high voltage paper electrophoresis. The major components identified were 2‐keto‐3‐deoxyoctonic acid, basic amino acids (free and bound), inorganic orthophosphate, ethanolamine phosphate and ethanolamine pyrophosphate. Ethanolamine pyrophosphate has not previously been found in acid hydrolysates of lipopolysaccharides. Ethanolamine phosphate and some of the orthophosphate were apparently produced by breakdown of ethanolamine pyrophosphate.