Open AccessThe Linkage of Pyrophosphorylethanolamine to Heptose in the Core of Salmonella minnesota Lipopolysaccharides
Author(s) -
Lehmann Volker,
LÜDERITZ Otto,
Westphal Otto
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01474.x
Subject(s) - heptose , hydrolysis , lipopolysaccharide , salmonella , mutant , chemistry , acid hydrolysis , biochemistry , fraction (chemistry) , degradation (telecommunications) , bacteria , biology , chromatography , gene , genetics , endocrinology , telecommunications , computer science
Mild acid hydrolysis of the P + lipopolysaccharide of Salmonella minnesota mRz, a UDP‐glucose synthetase‐less mutant, leads to the formation of 5 degradation products whose structures were studied. Free 2‐keto‐3‐deoxyoctonate (KDO), KDO‐7‐phosphorylethanolamine, and fraction P − (Hep‐Hep‐KDO) have been identified previously as degradation products of a P − lipopolysaccharide (glycolipid). Fractions P + (Hep‐(Hep‐4‐phosphate)‐KDO) and PPN (Hep‐(Hep‐4‐pyrophosphorylethanolamine)‐KDO) occur only in the P + mutant. It was shown that fraction P + is not an artifact which was formed from fraction PPN during partial hydrolysis. The simultaneous occurrence of fractions P + and PPN reflects microheterogenicity of the lipopolysaccharide. Possible reasons for this are discussed.