Open AccessCharacterization of the Messenger Ribonucleoprotein Released from Reticulocyte Polyribosomes by EDTA Treatment
Author(s) -
Lebleu Bernard,
Marbaix Gérard,
Huez Georges,
Temmerman Jacques,
Burny Arsène,
Chantrenne Hubert
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01313.x
Subject(s) - ribonucleoprotein , reticulocyte , polysome , messenger rna , messenger rnp , ribosome , ribosomal rna , rna , protein biosynthesis , biology , biochemistry , ribonucleoprotein particle , globin , chemistry , microbiology and biotechnology , gene
When reticulocyte polyribosomes are dissociated by EDTA treatment, two ribonucleoproteins are released: one of these, messenger ribonucleoprotein, contains the messenger RNA for globin chains and two heavy proteins (molecular weights 130 000 and 68 000); the other contains the 5 S ribosomal RNA and a lighter protein (molecular weight 45000). The protein moiety of the messenger ribonucleoprotein is required for the binding of the mRNA to deoxycholate‐washed native 40 S ribosomal subunits.