Open AccessStudies on the Structure of T1 Lipopolysaccharides
Author(s) -
Berst Mireille,
Lüuderitz Otto,
Westphal Otto
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01251.x
Subject(s) - periodate , galactose , ribose , chemistry , trisaccharide , lipopolysaccharide , polysaccharide , biochemistry , sodium periodate , periodic acid , stereochemistry , organic chemistry , biology , enzyme , endocrinology
Lipopolysaccharides of Salmonella T1 forms contain ribose and galactose both present as furanosides. Mild periodate oxidation of the T1 lipopolysaccharide followed by treatment with alkali resulted in the formation of a main fragment which was identified as poly‐ribose linked to the core polysaccharide. Poly‐ribose was also identified as a degradation product of periodate oxidized T1 lipopolysaccharide which had been treated with mild acid.Another fragment formed under these conditions was identified as being derived from ‐6‐Gal f ‐1,3‐Gal f ‐1,3‐Gal f ‐1,6‐Gal f ‐1‐. These results indicated that the T1‐specific polysaccharide of T1 lipopolysaccharides contain poly‐ribose and poly‐galactose regions. Galactose‐trisaccharide units may be present as repeating units of a poly‐galactose chain.