Open AccessTwo types of Pyruvate Kinase in Mucor rouxii
Author(s) -
Terenzi Héctor F.,
Roselino Eduardo,
Passeron Susana
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01249.x
Subject(s) - pyruvate kinase , pkm2 , biochemistry , pyruvate dehydrogenase phosphatase , yeast , mycelium , pyruvate dehydrogenase kinase , kinase , fructose , pyruvate decarboxylation , pyruvate dehydrogenase lipoamide kinase isozyme 1 , biology , pyruvate dehydrogenase complex , enzyme , chemistry , botany , glycolysis
Extracts of mycelium of Mucor rouxii have been shown to contain two forms of pyruvate kinase named Type I and II. They can be separated by DEAE‐cellulose chromatography and by electrophoresis in polyacrylamide gel. Extracts from ungerminated sporangiospores or yeast‐like cells contained only the Type I form. Pyruvate kinase Type II appears during aerobic germination of spores or during conversion of yeast‐like cells to mycelium. Both pyruvate kinases are similar in their molecular weight, pH range of activity, K m with respect to substrates and their response to activators (Mn ++ and fructose 1,6‐diphosphate).