Open AccessEvidence of an Enzymatic Degradation of Insulin in Blood in vitro
Author(s) -
Brodal Björn P.
Publication year - 1971
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1971.tb01231.x
Subject(s) - trichloroacetic acid , haemolysis , insulin , enzyme , degradation (telecommunications) , in vitro , chemistry , biochemistry , chromatography , medicine , biology , immunology , computer science , telecommunications
Haemolyzed red cells from human and rat blood possess the ability to split enzymastically the insulin molecule into its A and B chain without further degradation. The splitting of insulin incubated with different blood preparations, has been studied as a function of the degree of haemolysis, by the use of a trichloroacetic acid precipitation technique using 125 I‐labelled insulin. Kinetic data are obtained using haemolysate as crude enzyme.