Open AccessInability of Escherichia coli B to Incorporate Added Deoxycytidine, Deoxyadenosine, and Deoxyguanosine into DNA
Author(s) -
Karlström H. Olle
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb01135.x
Subject(s) - deoxyadenosine , deoxyribonucleoside , deoxyguanosine , deoxycytidine kinase , thymidine phosphorylase , deoxyribonucleosides , biochemistry , thymidine , purine nucleoside phosphorylase , chemistry , adenosine deaminase , deoxycytidine , deoxyribonucleotide , microbiology and biotechnology , dna , biology , enzyme , purine , genetics , oligonucleotide , chemotherapy , gemcitabine
It is known that Escherichia coli can incorporate labeled thymidine into its DNA, but it has not been previously possible to measure the incorporation of the other deoxyribonucleosides deoxycytidine, deoxyadenosine, and deoxyguanosine because of degradation of these compounds. The present experiments utilize a mutant of E. coli B lacking deoxyribonucleoside‐catabolizing enzymes. This strain, OK441, has mutations affecting the following enzymes: thymidine phosphorylase, purine nucleoside phosphorylase, eytidine deaminase, and adenosine deaminase. The results with OK441 show that E. coli , in contrast to lactobacilli and mammalian cells, does not incorporate any deoxyribonucleoside other than thymidine into its DNA. E. coli may be unable to phosphorylate deoxyeytidine, deoxyadenosine, and deoxyguanosine. In agreement with this suggestion, only thymidine kinase but no other deoxyribonucleotide kinase could be detected in extracts of OK441.