Open AccessPurification of Animal Neurotoxins
Author(s) -
Miranda François,
Kupeyan Charles,
Rochat Hervé,
Rochat Catherine,
Lissitzky Serge
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb01111.x
Subject(s) - sephadex , venom , chromatography , size exclusion chromatography , chemistry , amberlite , neurotoxin , amino acid , biochemistry , organic chemistry , enzyme , adsorption
The purification of eleven neurotoxins from the venom of three scorpions ( Androctonus australis Hector, Buthus occitanus tunetanus and Leiurus quinquestriatus quinquestriatus ) has been performed by Sephadex G‐50 filtration (with and without recycling) and equilibrium chromatography on Amberlite CG‐50, CM‐Sephadex C‐50 and DEAE‐Sephadex A‐50. All the chromatographic steps were carried out in ammonium acetate buffers. Purity of the neurotoxins was assessed by amino acid analysis and zone electrophoresis. All have a molecular weight of about 7000 daltons and do not contain methionine. As shown by amino acid composition and chain length scorpion neurotoxins show a high degree of homology.