Open AccessThe Nucleotide Sequences of Tyrosine Transfer RNAs of Escherichia coli
Author(s) -
Goodman H. M.,
Abelson J. N.,
Landy A.,
Zadrazil S.,
Smith J. D.
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb00950.x
Subject(s) - transfer rna , nucleotide , tyrosine , escherichia coli , nucleic acid sequence , biology , gene , microbiology and biotechnology , biochemistry , genetics , rna
The tyrosine tRNAs specified by the E. coli amber suppressor gene su III + and the wild type non‐suppressing allele su III − were selectively labelled with 32 P in E. coli infected with transducing phage ψ80 carrying either of these genes. The determination of the nucleotide sequence of the su III + tyrosine tRNA is described. This tRNA has the anticodon CUA. The su III − tRNA differs from su III + tRNA by a single nucleotide and has the anticodon where is a modified guanylic acid residue. E. coli su − cells contain two tyrosine tRNAs (I and II). The determination of the nucleotide sequence of these is described. Species I is identical to su III − tRNA while species II differs by two nucleotides in the variable loop region.