Open AccessThe Nucleotide Sequence of Methionine Transfer RNA M
Author(s) -
Cory Suzanne,
Marcker K. A.
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb00836.x
Subject(s) - methionine , transfer rna , uridine , nucleotide , ribonuclease , biochemistry , ribonuclease t1 , chemistry , rna , sequence (biology) , rnase p , biology , amino acid , gene
The species of methionine tRNA which places methionine into internal positions of growing polypeptide chains, methionine tRNA M , was purified from Escherichia coli strain CA265 labelled with 32 P by column chromatography on DEAE‐Sephadex and benzoylated DEAE‐cellulose. Sequence analysis of the products of complete and partial digestion of tRNA M by ribonuclease T 1 and by pancreatic ribonuclease permitted the derivation of the total primary structure of this molecule. The sequence of methionine tRNA M is where U * is probably 4‐thio‐uridine, and , A * and X are unknown nucleotides.