Open AccessThe Quantitative Measurement of DNA Hybridization from Renaturation Rates
Author(s) -
Ley J. De,
Cattoir H.,
Reynaerts A.
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb00830.x
Subject(s) - dna , dna–dna hybridization , measure (data warehouse) , similarity (geometry) , biology , chemistry , biological system , computational biology , genetics , computer science , data mining , image (mathematics) , artificial intelligence
A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture. The equations, relating the degree of binding with renaturation rates, are calculated for several cases. The optimal conditions for measurements are given. The method was checked in several ways. The advantages are summarized.