Open AccessChemically Modified Phenylalanine Transfer Ribonucleic Acid from Yeast
Author(s) -
Schlimme Eckhard,
Haar Friedrich,
Eckstein Fritz,
Cramer Friedrich
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb00296.x
Subject(s) - phenylalanine , transfer rna , adenosine , biochemistry , aminoacylation , chemistry , yeast , stereochemistry , rna , amino acid , gene
Adenosine 5′‐ O ‐(1‐thiotriphosphate) was incorporated in tRNA Phe ‐C‐C by C‐C‐A pyrophosphorylase. The resulting tRNA Phe ‐C‐C s̄ A could be charged with phenylalanine by aminoacyl‐tRNA synthetase and pyrophosphorolyzed with C‐C‐A pyrophosphorylase. Degradation with snake venom phosphodiesterase, however, was markedly reduced. Adenosine 5′‐ O ‐(1‐thiotriphosphate) did not substitute for ATP in the activation of phenylalanine by aminoacyl‐tRNA synthetase, but was a competitive inhibitor.