Open AccessStructural Investigations on the 2‐Keto‐3‐Deoxyoctonate Region of Lipopolysaccharides
Author(s) -
Dröge Wulf,
Lehmann Volker,
Lüderitz Otto,
Westphal Otto
Publication year - 1970
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1970.tb00276.x
Subject(s) - periodate , glycolipid , chemistry , lipid a , polysaccharide , hydrolysis , acid hydrolysis , biochemistry , mutant , salmonella , trisaccharide , bacteria , stereochemistry , biology , gene , genetics
Mild acid hydrolysis of the glycolipid derived from a Salmonella Rd 2 mutant led to the liberation of three major split products which, after separation by electrophoresis, were identified as free 2‐keto‐3‐deoxyoctonate (KDO), KDO‐7‐phosphorylethanolamine, and l ‐glycero‐α‐ d ‐mannoheptosyl‐1,5‐KDO. By the use of various methods of KDO determinations, and by periodate oxidation performed on the glycolipid, results were obtained which indicate that the bridge between the polysaccharide portion of lipopolysaccharides and lipid A is formed by a branched KDO trisaccharide whose KDO side chain is randomly substituted by phosphorylethanolamine according to the formula —Hep‐1,5‐KDO‐KDO‐lipid A | KDO‐(phosphorylethanolamine) Analogous results were obtained with P‐lipopolysaccharides of other Salmonella mutants.