Open AccessBiochemical Studies on Lipopolysaccharides of Salmonella R Mutants
Author(s) -
Gmeiner J.,
Lüderitz O.,
Westphal O.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb19618.x
Subject(s) - heptose , chemistry , glucosamine , hydrolysis , biochemistry , acid hydrolysis , glycolipid , lipid a , borohydride , ninhydrin , glycosidic bond , salmonella , galactose , sialic acid , mutant , enzyme , bacteria , amino acid , biology , catalysis , genetics , gene
The endotoxically active cell wall glycolipid of a highly deficient heptose‐less R mutant of Salmonella minnesota , consisting of 2‐keto‐3‐deoxyoctonic acid (KDO) and lipid A, was subjected to hydrazinolysis. Besides fatty acid hydrazides, a water‐soluble main fraction was obtained, containing KDO, glucosamine, and ester phosphate and free phosphorylethanolamine. The application of two procedures of partial hydrolysis, followed by paper electrophoretic separation, led to seven oligosaccharides. Their structures were evaluated with the aid of the following analytical methods: further acid degradation, borohydride reduction, ninhydrin degradation, the Morgan‐Elson reaction, treatment with phosphomonoesterase and N ‐acetylglucosaminidases. The results show that the backbone of lipid A contains phosphorylated glucosaminyl→glucosamine disaccacharide units, probably linked β‐1,6, to which a trisaccharide composed of three KDO residues is linked ketosidically to form a pentasaccharide which appears to be a common structure in many Enterobacteriaceae .