Open AccessNature of RNA Synthesized at Low and High Ionic Strength by DNA‐Dependent RNA‐Polymerase from Escherichia coli
Author(s) -
Qasba P. K.,
Zillig W.
Publication year - 1969
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1969.tb19609.x
Subject(s) - ionic strength , rna , rna polymerase , dna , ionic bonding , escherichia coli , chemistry , polymerase , biology , biophysics , biochemistry , microbiology and biotechnology , ion , gene , organic chemistry , aqueous solution
By competition hybridization experiments it is shown, that RNA synthesized in vitro by RNA polymerase on T 4 ‐DNA at high ionic strength, like that synthesized at low ionic strength, contains only early RNA species. However, the product obtained at high ionic strength contains sequences not synthesized at low ionic strength. This is attributed to the early arrest of synthesis at low ionic strength.